简论犬卵母细胞体外成熟和冷冻保存基础

论文导读：oundssupplementation20-21Chapter2Theinfluenceof9-cis-retinoicacidonnuclearandcytoplaicmaturationandgeneexpressionincanineoocytesduringinvitromaturation21-312.1Introduction21-222.2Materialsandmethods22-262.2.1Collectionofcanineovariesandcumulus-oocytecomplexes222.2.2
摘要：关键词：
【学位授予单位】：论文延边大学
【学位级别】：论文硕士
【学位授予年份】：论文2012
【分类号】：论文S829.2
【目录】：论文Abstract6-8
Table of Content8-12
Chapter 1 Research background12-21

1.1 Introduction12

1.2 Review of the pterature12-15

1.2.1 Reproduction in the bitch12-13

1.2.2 Endocrine characteristics13-14

1.2.3 Ovarian folpculogenesis,oogenesis and oocyte maturation14-15
1.2.3.1 Ovarian folpculogenesis and oogenesis14-15

1.2.3.2 Ovulation and oocyte maturation15

1.3 Factors influencing in vitro maturation of canine oocytes15-21
1.3.1 Oocyte size,age and reproductive cycle of bitch and folpcle size15-16
1.3.2 Culture condition for IVM of canine oocytes16-18

1.3.2.1 Culture density16-17

1.3.2.2 Co-culture17

1.3.2.3 Culture time17

1.3.2.4 Oxygen tension and temperature17-18

1.3.2.5 Culture medium18

1.3.3 The influence of medium supplementation on the IVM of canine oocytes18-21
1.3.3.1 Growth hormone and growth factors supplementation18-19

1.3.3.2 Protein supplementation19-20

1.3.3.3 Antioxidants and energy substrates supplementation20
1.

3.4 Other compounds supplementation20-21

Chapter 2 The influence of 9-cis-retinoic acid on nuclear and cytoplaic maturation and gene expressionin canine oocytes during in vitro maturation21-31

2.1 Introduction21-22

2.2 Materials and methods22-26

2.2.1 Collection of canine ovaries and cumulus-oocyte complexes22

2.2 In vitro maturation22-23

2.2.3 Removal of cumulus cells and assesent of oocyte nuclear maturation23-24
2.2.4 Labepng of oocytes with fluorescein isothiocyanate(FITC)-lectins for cortical granule(CG) and chromosomal staining24-25
2.2.5 Gene expression of canine cumulus cells25-26

2.6 Statistical analysis26

2.3 Results26-28

2.4 Discussion28-30

2.5 Conclusion30-31

Chapter 3 Influence of ovarian cortex cells co-culture during论文导读：-464.1Introduction38-394.2Materialsandmethods39-424.2.1Chemicals394.2.2Collectionofcanineovaries,oocytesandovariantissues39-404.2.3Vitrificationandthawingofoocytes40-414.2.4Vitrificationandthawingofovariantissues414.2.5Analysisofoocytevitrified-warmedviabipty41-424.2.6St
in vitro maturation on meiotic resumption ofcanine oocytes31-38

3.1 Introduction31

3.2 Materials and methods31-34

3.

2.1 Chemicals and reagents31

3.2.2 Collection of canine ovaries and COCs31-32
3.2.3 Preparation of canine ovarian cortex cells(COCCs)monolayers32-33
3.

2.4 In vitro maturation33

3.2.5 Removal of cumulus cells and assesent of oocyte nuclear maturation33-34
3.

2.6 Statistical analyses34

3.3 Result34-35

3.4 Discussion35-36

3.5 Conclusion36-38

Chapter 4 Canine oocyte collection during ryopreservation of the ovarian tissues38-46

4.1 Introduction38-39

4.2 Materials and methods39-42

4.

2.1 Chemicals39

4.2.2 Collection of canine ovaries,oocytes and ovarian tissues39-40
4.2.3 Vitrification and thawing of oocytes40-41
4.2.4 Vitrification and thawing of ovarian tissues41
4.2.5 Analysis of oocyte vitrified-warmed viabipty41-42
4.

2.6 Statistical analysis42

4.3 Results42-43

4.4 Discussion43-45

4.5 Conclusion45-46

Acknowledgments46-47
Reference47-59
Appendix A59-60
Appendix B60